2014-02-27
In the presence of ABA, ABI5 levels increase drastically via a decrease in ubiquitin-meditated proteasomal degradation. Our results indicate that ABA promotes ABI5 accumulation by inducing the ubiquitination and proteasomal degradation of KEG.
However, the precise role of barley (Hordeum vulgare) ABI5 in ABA signaling and its function under stress remains elusive. Here, we show that HvABI5 is involved in ABA-dependent regulation of barley response to drought The accumulated ABI5 protein further shows heteromeric interaction with HY5, and thus synergistically activates its own expression. Our findings reveal a novel transcriptional switch, composed of JMJ17–WRKY40 and HY5–ABI5 modules, which regulates the ABA response during seed germination and seedling development in Arabidopsis. ABA and GA can regulate ABI5 activity coordinately during seed germination and early seedling development (Piskurewicz et al., 2008). Recent reports suggest that ABA represses the growth of the embryonic axis through enhanced auxin signaling, revealing the interaction between ABA and auxin signaling pathways (Belin et al., 2009).
Moreover, ABI5 also acts as an ABA and other phytohormone signaling integrator. Components of auxin, cytokinin, gibberellic acid, jasmonate and brassinosteroid signaling and metabolism pathways were shown to take part in ABI5 regulation and/or to be regulated by ABI5. Monocot orthologs of AtABI5 have been identified. In addition, in the presence of exogenous ABA, the abi5 pho1 double mutant, similar to the pho1-2 mutant, showed significantly slower and the abi5 mutant displayed increased germination rates compared with wild-type plants . These results demonstrate that PHO1 is epistatic to ABI5 in ABA-mediated seed germination and early seedling development. ABA INSENSITIVE 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor which acts in the abscisic acid (ABA) network and is activated in response to abiotic stresses.
2020-03-02 · Moreover, we observed that on 0.3 μM ABA, growth of abi5 pifq mutant seedlings was more resistant to ABA than that of pifq mutants, but similar to that of abi5 (Figure 4D). These observations indicate that ABI5 is epistatic to PIFs in mediating hypocotyl-negative gravitropism and ABA signaling in darkness. In addition, ABI5 was shown to directly activate its own expression, whereas BBX21 negatively regulates this activity by directly interacting with ABI5.
När miljöfaktorer som temperatur inte är optimala för grodd av frö är ABA-nivåer höga, vilket orsakar produktion av högre nivåer av ett protein som kallas ABI5.
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Further, we used a genetic approach taking advantage of the weak aba insensitive genes which are affected by DOG1 partly via control of ABI5 expression.
PLoS Genetics.
The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. ABI5 functions upstream of miR156 to promote juvenile development by affecting the expression of genes in the miR156-SPL pathway. Therefore, our study uncovers a new role of ABI5 in vegetative development in plants, and implies a role of ABA signaling in vegetative development in Arabidopsis. Our genetic data show that BBX21 may act upstream of several ABA INSENSITIVE (ABI) genes and ELONGATED HYPOCOTYL 5 (HY5) in ABA control of seed germination. Previous studies showed that HY5 acts as a direct activator of ABI5 expression, and that BBX21 interacts with HY5.
The constitutive expression of ABI5 prevented the growth arrest of ppc2 under low CO2 conditions. These findings demonstrate that PPC2 plays an important role in the acclimation of Arabidopsis plants to low CO2 availability by linking photorespiratory metabolism to primary metabolism, and that this is mediated, at least in part, through ABA and
The transcription factor ABI5 is an important regulator in the ABA signaling pathway. ABI5 confers enhanced responses to exogenous ABA during seed germination: the abi5 mutant shows obvious ABA-insensitive phenotypes (Piskurewicz et al., 2008), whereas ABI5-overexpressing lines are ABA sensitive (Piskurewicz et al., 2008; Bu et al., 2009).
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ABI5 functions in the core ABA signaling, which is composed of PYR/PYL/RCAR receptors, PP2C phosphatases and SnRK2 kinases, through the 2018-02-20 · ABA induces a number of effectors, including the bZIP transcription factor ABA INSENSITIVE5 (ABI5). ABI5 accumulates during seed maturation and in dry seeds [19, 20]. During the normal course of seed germination, ABA and concomitantly ABI5 levels rapidly decline following imbibition and GA biosynthesis, enabling seed germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a We used cDNA microarrays to analyze ABA regulated gene expression and the role of ABI5 in seedlings.
Studies have identified the key components of ABA signaling in Arabidopsis (Arabidopsis thaliana), some of which regulate ABA responses by the transcriptional regulation of downstream genes. Here, we report the functional identification of rice (Oryza sativa) ABI5-Like1 (ABL1), which is a basic region/leucine zipper motif transcription factor. However, ABI5 was found to modulate the PYL11‐ and PYL12‐mediated ABA response. In the abi5‐7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked.
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mutants, the abi5-1 mutants had lower photosynthetic rates and Fv/Fm compared to the wild type under photorespiratory conditions i.e. low CO2 availability. However, the growth of these mutants was similar to the wild type under non-photorespiratory (low O2) conditions. The constitutive expression of ABI5
Over-expression of AFP1 enhances tolerance to ABA and reduces ABI5 accumulation, whereas afp1 mutants are sensitive to ABA and have high levels of ABI5 (Lopez-Molinaetal.,2003).TherearethreeAFP1homologs(i.e. AFP2, AFP3, and AFP4) in the Arabidopsis genome, 2001-04-10 Comparison of seed and ABA-inducible vegetative gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages. Responsible for reducing cadmium uptake, mediated by interaction with MYB49 .